(945)248-0060 / (469)815-2898 office@novavitarepro.com 11162 Osburn Road, Pilot Point, TX, USA

Every Stage of the ICSI cycle carefully tailored to your specific needs

Whether you have an aged mare or a top-level performer we are here to help make the process run smoothly. We have a fee schedule tailored to protect your bottom line and transparent communication throughout the process to give you valuable insight at every step.

Getting started with ICSI

Getting started with ICSI

Vita Nova is a LABORATORY only. We work closely with outside veterinarians and recipient farms throughout the process. First step is to locate a skilled, licensed veterinarian or breeding manager in your area. The initial goal is to determine and track your mare's follicular development. This will determine the best time to perform a trans-vaginal oocyte aspiration (TVA), also known as oocyte Pick-Up (OPU) — which is an ultrasound-guided retrieval of oocytes (eggs) directly from the mare's ovaries.

*Please be aware that each facility charges a different fee for these services and they are not associated with Vita Nova's costs and procedures.*

Once your mare is ready for aspiration, you, the attending veterinarian or your reproduction manager will need to contact Taryn, our office manager, at (469)815-2898 to book your mare on our calendar. We will need our contracts (found on our website under Contracts & Fees) on file at this time.

You will need to have all stallion contracts finalized and semen in place at our laboratory prior to the arrival of your oocytes. We have many stallions already on-hand to help simplify this step. Please contact Taryn if you have questions about the availability of specific stallions.

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Intracytoplasmic Sperm Injection (ICSI)

Intracytoplasmic Sperm Injection (ICSI)

The ICSI procedure involves taking a single sperm cell and injecting it directly into mature oocytes from your mare under a microscope. This process makes it possible for us to use sub-fertile stallion semen, to conserve limited straws to stretch the useable life of individual stallions, and to obtain foals from aged or difficult mares. ICSI also makes it possible to obtain embryos from mares repeatedly throughout the year, as TVA/OPU does not require the mare to be in heat.

Once oocytes are retrieved from your mare by your veterinarian, they are shipped via FedEx, UPS, courier, or counter-to-counter services to our lab. Oocytes then undergo a maturation process for approximately 30-36 hours. Once mature, these oocytes are each individually injected with a single sperm cell from the stallion of your choice. Please know that not all oocytes sent from your mare will mature for this process. On average, we expect 50-60% to mature to be fertilizable.

Once fertilized, the Zygotes (fertilized embryos) are placed into our culture system for a 10-day period. We do not interrupt them in culture, except to check for cleavage at day 5. Cleavage is a marker that tells us that the oocytes were successfully fertilized and are continuing development in our system. They are NOT a useable embryos just yet.

Embryo evaluation begins on Day 7-Day 10 post ICSI. We consistently work with the recipient or veterinary facility of your choice to coordinate the fresh transfer of your developing embryos to a recipient mare. If you have multiple embryos or are not quite ready to transfer them, as is the case in the fall, we can vitrify (freeze) them for later use.

Please see our fee schedule on the Contracts & Fees page for an outline of the costs of these services.
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Embryo Transfer & Recipient Mares

Embryo Transfer & Recipient Mares

For performance and show mares who need to stay in competition, or for older/sub-fertile mares who struggle to carry, a recipient mare becomes necessary to carry foals. Whether you prefer to buy your own or lease them, we are happy to coordinate with you to increase your likelihood of success.

There is a lot to be said about utilizing an outside facility that has a professionally maintained herd of healthy, cycle-synchronized, proven recipient mares — allowing you to keep breeding, showing, or competing without interrupting your season. We have several facilities that we have worked with over the years with fantastic rates that we would love to point you to. While this may seem like the more expensive option, when you factor all the costs of feeding, farrier, teeth, vaccinations, hormone injections, palpations, and transfer costs- a recipient mare adds up.

We have typically seen lower pregnancy rates for personally owned recipient mares, usually due to poor synchrony, body condition, unknown fertility, lack of uterine preparation, and less skilled transfer techniques. Saving $2,500 on the cost of a mare may not be worth it if one of these factors leaves you with an open vessel to feed. If you are able to purchase your own recipient mares and have them closely managed by a skilled reproduction veterinarian, you likely will see higher pregnancy rates.

Please know we support you either way you go, but we want you to have the best possible chance at a foal. We are always happy to recommend facilities and managers we have had good success with if you need help. (Please know that we do not receive any kind of remuneration for our referrals)

Embryos can also be vitrified (frozen) for transfer at a later date, and we have many outside facilities capable of warming our embryos mare-side for transfer to increase your chances of success.

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Embryo Vitrification

Embryo Vitrification

Whether you had a recipient mare not line up or work out for your flushed embryo, or you are just doing fall ICSI to get a jump-start on spring transfers, we are able to handle the cryopreservation and short-term storage of your embryos. This allows you to be better positioned for a successful outcome.

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Embryo Storage

Embryo Storage

*Please note, we are NOT a long-term storage facility* We are happy to store frozen embryos produced from your ICSI sessions for a season or two following their production, but strongly encourage you to move embryos to a longer-term facility such as Select Breeders, if you are not planning to transfer anytime soon

Our Storage fee is $50 per QUARTER, or $200/year for each mare/stallion cross, or sperm sent for owner-only use. We do require a frozen commodities agreement to be on file for any genetic material kept in storage over 3-months.

Please note that it is the owner's responsibility to insure their genetic material against damage or loss while in our care.

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Post-Mortem Ovary Harvest

Post-Mortem Ovary Harvest

Our team is highly experienced in Post-mortem ovary harvest. We are able to coach your veterinarian through ovary removal and shipment to our lab, where we take meticulous care to ensure the highest possible recovery rate per available follicle. Ensuring your mare's legacy is carried forward is our top priority.
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A Clear, Guided Path From First Call to Healthy Foal

We believe you should always know what happens next. Here is the typical path most clients follow.

01

Consultation & Goal Review

We start with a call or visit to discuss your mare's history, your goals for the season, your location, and professionals to work with in your area to streamline the process

02

Performance of Oocyte aspiration

You work with your designated veterinarian or reproduction manager to have your mare aspirated and oocytes sent to our lab for processing. A contract is placed on file with us at this time for your mare's work. No up-front fees or deposits are required

03

Semen & ICSI

If semen from your desired stallion is not already available in our lab, Taryn, our office manager will coordinate this for you! We handle all the coordination and communication so that you can sit back and trust you are in good hands. ICSI occurs on the second day after your mare is aspirated.

04

Embryo Development

At day FIVE post-ICSI, you will receive an update on development. Embryos that can be transferred or vitrified are noted between days 7-9 post-ICSI. We coordinate directly with your veterinarian or recipient farm to facilitate transfer anywhere across the country and you will always know what is happening in real time.

05

Embryo quality and reporting

If we feel an embryo we produced may be sub-par, or if we have any indication it may not make a pregnancy, this information is shared with both you and the stallion owner/manager. We often DO NOT charge for any embryos we do not suspect have a high probability of working, leaving the choice to freeze/transfer/or discard up to you.

Advanced Level Teamwork

There are many different ICSI laboratories now, but our dedicated care, exceptional results, and tedious reporting are often quoted as some of the reasons many clients switched to using us.

CapabilityVita Nova Reproduction CenterTypical General Practice
Clearly communicated options at the time of ICSI Options given for stallion splits or ICSI-opt out if numbers are low ICSI is auto performed without consulting the client first
Transparent embryo reporting Lower embryo grade is ALWAYS shared and options given, including a no-charge Little to no reporting, No option to keep or discard given, and full fees are charged
Discounts and credits Half-price vitrification fee for multiples, multiple mare discounts, and loyalty program to reward regular clients Same price charged no matter how many are frozen, no added discounts, no loyalty perks

Answers Before You Book

What is the difference between AI, embryo transfer, and ICSI?

Artificial Insemination (AI) places semen, either fresh or frozen, directly into a mare's uterus around ovulation — it is the most common and least invasive option, and produces a foal with the genetics of the mare being inseminated. Embryo Transfer moves a fertilized embryo from a donor mare into a recipient mare, letting a performance mare stay in competition, or an aged or difficult mare that is unable to carry still continue to produce foals. ICSI (Intracytoplasmic Sperm Injection) is a laboratory technique where a single sperm is injected into a single egg that was recovered from a donor mare via an OPU procedure, under a microscope — it was initially developed for cases where semen quantity/quality was limited, or for mares unable to conceive or carry a pregnancy conventionally. Today, it is becoming a common replacement for traditional embryo transfer due to the fact that the donor mare does not need to be cycling to have an OPU to remove her oocytes (eggs) performed, the cost is often very similar to standard ET, and there is often the potential for multiple embryos to be produced, while traditional methods often only offer an opportunity to recover one or two if lucky.

What is Oocyte Pick-Up (OPU) and when is it recommended?

OPU is an ultrasound-guided procedure where we retrieve immature eggs directly from a mare's ovaries, without needing her to be in heat. It was originally recommended for mares that cannot carry a pregnancy, have failed conventional breeding attempts. Now it is additionally useful for mares in competition or for those with exceptional pedigrees and show records that are booked to multiple stallion contracts in a year. In the off-season, ICSI is utilized to bank a number of embryos and to satisfy stallion contracts that did not result in an earlier pregnancy so that mare owners can avoid paying additional fees. Having embryos banked in the fall allows mare owners to start earlier in the season with their transfers.

Do you guys perform oocyte aspiration?

No- we work directly with veterinarians across the country who aspirate and ship the oocytes to us. We have several great veterinarians we can refer you to for this portion of the procedure and we are happy to work directly with them to streamline the process and if possible, reduce shipping costs for you! Call us today to find out more

Do you guys have recipient mares and do the embryo transfer?

No- we do not have recipient mares at our facility and we do not perform embryo transfer. However, we work closely with many advanced reproductive veterinarians and top-tier breeding farms in the area, and have a slew or great facilities and repro specialists we can refer you to within a short drive of our place.

How many ICSI cycles does it usually take to get a mare in foal?

As with standard reproductive techniques, healthy, fertile mares and stallions often have higher success rates early in the process, while aged or challenge mares, or stallions utilizing twice-refrozen semen, may require multiple attempts to obtain a working pregnancy.

Do you work with only frozen semen?

No! While we primarily utilize frozen semen due to its ease of transport and ready availability, we do still use cooled semen when we can obtain it for ICSI purposes. We actually prefer cooled when possible, as it can sometimes give a higher embryo rate.

My mare is still competing — can she still have a foal this year?

Yes — this is a great scenario where ICSI can be beneficial. Your mare simply needs an ultrasound with your reproductive veterinarian to determine if/when she is a good aspiration candidate. She does not need to be in heat or pulled from competition for lengthy breeding protocols. Following aspiration, your mare can return to competition as early as the next day, barring any negative side-effects. Once embryos are made via ICSI, they can be sent for transfer into a proven recipient mare managed at a professional facility. If all goes well, the recipient mare carries the pregnancy while your mare continues training and showing.

Do you board mares during the breeding season?

While we do have access to two ten-stall barns on our facility, and our team has ample experience managing mares for reproductive procedures, we do not openly offer this service at this time. However, if you are a long-standing client, or a client in a state with little to no advanced reproductive vets in your area, feel free to contact us to discuss the private option of having your mare managed at our facility for oocytes aspiration & ICSI. This would be done with the skilled veterinary team at Weems & Stephens Equine Hospital in Aubrey, TX.

Not Sure Where to start?

Every mare is different, and not all situations are an easy one and done. Contact our team for a reproduction plan and network of professionals dedicated to meeting your specific goals.